Assay of proteolytic enzyme(s) from Neurospora crassa
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چکیده
منابع مشابه
Protoplasts from Neurospora crassa.
Weibull (1953) demonstrated that the intact protoplasts are liberated from cells of Bacillus megaterium after digestion of the cell wall by the enzyme lysozyme, and that these structures persist and exhibit many of the activities of the whole cell provided they are maintained in solutions of appropriate osmotic pressure. Since that time free protoplasts have been obtained from many other gram-p...
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The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...
متن کاملEnzymes of deoxythymidine triphosphate biosynthesis in Neurospora crassa mitochondria.
Intact mitochondria of Neurospora crassa incorporate deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid but not the label from (methyl-3H) deoxythymidine. Mitochondrial homogenates contain deoxythymidylate kinase (EC 2.7.4.9), deoxycytidylate aminohydrolase (dCMP deaminase) (EC 3.5.4.12), and thymidylate synthetase (EC 2.1.1b), but not thymidine kinase (EC 2.7.1.21) activity. dTM...
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Neurospora crassa colonizes burnt grasslands in the wild and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secretion of a wide variety of genes encoding lignocellulolytic enzymes. However, the means by which N. crassa and other filamentous fungi sense ...
متن کاملMOLECULAR ANALYSIS OF THE SULFUR REGULATORY CIRCUIT OF NEUROSPORA CRASSA
The sulfur regulatory circuit of the filamentous fungus, Neurospora crassa, consists of a set of unlinked structural genes which encode sulfur catabolic and two major regulatory genes which govern their expression. The cys-3 regulatory gene encode a transacting regulatory protein which activates the expression of cys-14 and ars, whereas the other regulatory genes Scon-l and Scon-2 appear to...
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ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1965
ISSN: 1941-4765
DOI: 10.4148/1941-4765.2112